Any time I have a conversation with small molecule or metabolomics researchers, I immediately think about how much their toys are going to impact proteomics when the technology moves over. By toys, I mean the cool column chemistries, derivitizations, labels, etc., These technologies are essential to the studying of these smaller compounds but their usefulness to peptide analysis often hasn't been evaluated.
Case in point: This paper from Chen Ding & Jing Jiang et. al., out of the Beijing Proteomics Center that is currently in press at MCP. In this study, they used a novel 2 dimensional separation technique to rapidly separate complex proteomes. The coverage and speed is honestly a little unbelievable compared to what is currently out there. The authors report 8,000 unique proteins per 12 hours of run time, with a peak speed approaching 2,200 proteins per half hour on an Orbitrap Velos and 2,800 proteins per half hour on both a Q Exactive and a Q-TOF system.
The false discovery rate calculation scheme for these datasets is a bit on the unique side, but the authors do a good job of providing substantiating data to suggest that these IDs are real. Considering that the success of this separation protocol demonstrated on multiple systems, it should be something that we see rapidly adopted by many labs.
0 comments:
Post a Comment