In partial explanation of why I haven't been writing very much, the complete series of Proteome Discoverer 1.4 videos are now up on planet orbitrap. All of the videos are 5-15 minutes and directly address specific steps or processing methods
The list is as follows:
New in PD 1.4:
FASTA databases:
Configuring Mascot:
Getting started with the PD 1.4 Daemon:
Setting up/using spectral libraries:
Setting up a simple workflow:
False discovery (PSM validation) nodes:
Workflow templates (exporting/importing):
Using the annotator node:
Running an iTRAQ/TMT experiment:
Processing SILAC data:
Label free quan experiments (PIAD):
Processing neutral loss triggered MS2 LTQ data:
Processing neutral loss triggered MS2 Q Exactive data:
Exporting unmatched spectra for de novo or PTM analysis:
Installing MSAmanda:
Multi-enzyme processing in PD 1.4:
www.planetorbitrap.com\libraries
Be sure to watch them in HD or they're hard to see.
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