To deplete or not to deplete -- part 2
In another of my favorite proteomics controversies, a new depletion technique takes a swing at whether depleting plasma is a good idea or not.
In this study, Seong-Beom Ahn and Alamgir Khan look at the impact depleting plasma has on cytokine recovery. Since cytokines are generally pretty low abundance, it makes for a decent metric. The depletion they use is an immuno-based one using magnetic beads.
Unlike other methods, this pull down approach showed either no impact or improved recovery of the cytokines. Definitely worth checking out!
This is what you get if you Google "pug hamlet"
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